Abstract: Greek pre-service teachers? level of ocean literacy was assessed using a revised questionnaire concerning ocean content knowledge and an instrument about ocean stewardship. Rasch analyses showed that the items of both measures were well targeted to the sample. Pre-service teachers possessed a moderate knowledge of ocean sciences issues and positive attitudes toward ocean stewardship; they obtained most information on ocean content from the Internet and mass media and less from formal education, nongovernmental organizations, books, and out-of-school settings. Students who mostly preferred the Internet and mass media scored significantly higher on the knowledge questionnaire. The results could contribute to the enhancement of teachers? ocean literacy.